
Labo. (pièce 417)
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Thématiques de recherche
Imagerie calcique et comportement du poisson zèbre et Danionella CerebrumPrésentation
Multisensory signal processing: From whole-brain activity to behavior
Our brain needs to constantly fuse sensory information detected by our multiple senses in order to produce a seamless coherentre presentation of the world. Rather than being the exception, this binding process is ubiquitous to sensory-motor integration and isimplicated in most cognitive functions. Its impairment is a cause of various pathologies, such as schizophrenia or autism. Multisensoryprocessing operates on all brain levels from primary cortices over subcortical structures up to higher associative centers, while thesmallest operational units are single multisensory neurons.
In an interdisciplinary effort, we combine optical developments, genetics and neuro-computation to obtain new insights into the activityof brain-wide neural circuits that process multisensory information. To reduce the complexity, we study the small transparent brain ofzebrafish larvae as a model system. We focus on gaze stabilization as an inherently multisensory model task that is conservedamong all vertebrates. This reflex uses both vestibular and visual information to drive eye movements in order to compensate forself-motion and maintain clear vision. We have developed a novel experimental platform in which a restrained larva can be submittedto vestibular and visual stimuli, as a pilot in a flight simulator. We can optically record the activity of all 100,000 neurons of the animalbrain as it performs multisensory integration tasks.To extract basic principles of how behavior is coded in multisensory neuronal circuits we interpret the brain-wide activity and theobserved behavior with methods from statistical physics. No other system can today provide a similar brain-scale, yet cell-resolvedview on the neuronal network dynamics subserving such a complex integration process.
We use this system to address three fundamental and generic questions relevant to multi-sensory integration.
1 – Multi-sensory enhancement. How multi-modal congruent sensory cues are integrated at the circuit level to enhance the precisionof the evoked motor response?
2 – Decision-making: Resolving conflicting sensory cues. When the brain is submitted to conflicting stimuli, which neuronalmechanism controls the prioritization of one sensory cue over the other?
3 – Cross-modal adaptation. How constantly out-of-register stimuli induces network plasticity in order to recover a coherentrepresentation ?
Selected publication:
Whole-Brain Calcium Imaging during Physiological Vestibular Stimulation in Larval Zebrafish
Migault et al. Current Biology (2018)The microscope in action:
People:
Publications
2024
⊞ | Structure and individuality of navigation in zebrafish larvae - hal (Feb. 2024) |
2023
⊞ | Magnetic actuation of otoliths allows behavioral and brain-wide neuronal exploration of vestibulo-motor processing in larval zebrafish - Current Biology (Sep. 2023) |
URL | Full text PDF | Bibtex | doi:https://doi.org/10.1016/j.cub.2023.05.026 |
⊞ | Random-access two-photon holographic optogenetic stimulation combined with brain-wide functional light-sheet imaging in larval zebrafish - Advances in Microscopic Imaging IV (Sep. 2023) |
⊞ | Multimodal units fuse-then-accumulate evidence across channels - BioRxiv (Jul. 2023) |
⊞ | A Versatile and Open Source One- and Two-Photon Light-Sheet Microscope Design - BioRxiv (Jul. 2023) |
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Bibtex | doi:https://doi.org/10.1101/2023.07.10.548107 |
⊞ | Neural assemblies uncovered by generative modeling explain whole-brain activity statistics and reflect structural connectivity - ELIFE (Mar. 2023) |
2021
⊞ | Thermal modulation of Zebrafish exploratory statistics reveals constraints on individual behavioral variability - BMC Biology (Sep. 2021) |
URL | Full text PDF | Bibtex | doi:https://doi.org/10.1186/s12915-021-01126-w |
2020
⊞ | From behavior to circuit modeling of light-seeking navigation in zebrafish larvae - eLife (Jan. 2020) |
URL | Full text PDF | Bibtex | doi:10.7554/eLife.52882 |
2018
⊞ | Whole-Brain Calcium Imaging during Physiological Vestibular Stimulation in Larval Zebrafish - Current Biology (Nov. 2018) |
URL | Full text PDF | Bibtex | doi:https://doi.org/10.1016/j.cub.2018.10.017 |
2017
⊞ | Sensorimotor computation underlying phototaxis in zebrafish - Nature Communication (Sep. 2017) |
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Full text PDF | Bibtex | doi:10.1038/s41467-017-00310-3 |
2016
⊞ | Rheotaxis of Larval Zebrafish: Behavioral Study of a Multi-Sensory Process - Frontiers in System Neuroscience (Feb. 2016) |
URL | Full text PDF | Bibtex | doi:10.3389/fnsys.2016.00014 |
2015
⊞ | Hair-Bundle Friction from Transduction Channels' Gating Forces - MECHANICS OF HEARING: PROTEIN TO PERCEPTION (Jan. 2015) |
2014
⊞ | Transduction channels' gating can control friction on vibrating hair-cell bundles in the ear - Proc. Natl. Acad. Sci. U. S. A. (May. 2014) |
2013
⊞ | Kinesin-8 Is a Low-Force Motor Protein with a Weakly Bound Slip State - Biophys. J. (Jun. 2013) |
2012
⊞ | The Highly Processive Kinesin-8, Kip3, Switches Microtubule Protofilaments with a Bias toward the Left - Biophys. J. (Jul. 2012) |
2010
⊞ | Microtubule Dynamics Reconstituted In Vitro and Imaged by Single-Molecule Fluorescence Microscopy - MICROTUBULES, IN VITRO: MICROTUBULES, IN VITRO (Jan. 2010) |
⊞ | Studying Kinesin Motors by Optical 3D-Nanometry in Gliding Motility Assays - MICROTUBULES, IN VITRO: MICROTUBULES, IN VITRO (Jan. 2010) |
⊞ | Breaking of bonds between a kinesin motor and microtubules causes protein friction - OPTICAL TRAPPING AND OPTICAL MICROMANIPULATION VII (Jan. 2010) |
2009
⊞ | Kinesin-8 Motors Act Cooperatively to Mediate Length-Dependent Microtubule Depolymerization - Cell (Sep. 2009) |
⊞ | Protein Friction Limits Diffusive and Directed Movements of Kinesin Motors on Microtubules - Science (Aug. 2009) |
2008
⊞ | Optical trapping of coated microspheres - Opt. Express (Sep. 2008) |
⊞ | Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope - J. Neurosci. Methods (Aug. 2008) |
⊞ | Optical monitoring of neuronal activity at high frame rate with a digital random-access multiphoton (RAMP) microscope - J. Neurosci. Methods (Aug. 2008) |
2007
⊞ | LED illumination for video-enhanced DIC imaging of single microtubules - J. Microsc.-Oxf. (Apr. 2007) |
2004
⊞ | Injection and flow control system for microchannels - Lab Chip (Aug. 2004) |