Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity

K. Aubertin , S. Bonneau , A.K.A. Silva , J.C. Bacri , F. Gallet , C. Wilhelm

Bibtex , URL
PLOS One, 8, 12, UNSP e84850
Published 27 Dec. 2013
DOI: 10.1371/journal.pone.0084850
ISSN: 1932-6203


The intracellular microenvironment is essential for the efficiency of photo-induced therapies, as short-lived reactive oxygen species generated must diffuse through their intracellular surrounding medium to reach their cellular target. Here, by combining measurements of local cytoplasmic dissipation and active trafficking, we found that photosensitizers activation induced small changes in surrounding viscosity but a massive decrease in diffusion. These effects are the signature of a return to thermodynamic equilibrium of the system after photo-activation and correlated with depolymerization of the microtubule network, as shown in a reconstituted system. These mechanical measurements were performed with two intracellular photosensitizing chlorins having similar quantum yield of singlet oxygen production but different intracellular localizations ( cytoplasmic for mTHPC, endosomal for TPCS2a). These two agents demonstrated different intracellular impact.

Cette publication est associée à :

Plasticité membranaire et fonctions cellulaires