Toward nonlinear endomicroscopy for exploration of the pulmonary airways: preliminary spectroscopic study of human lung tissue using a single-mode fibre
D.A. Peyrot
,
C. Lefort
,
D.A. Haidar
,
T. Mansuryan
,
S. Kruglik
,
N. Sandeau
,
F. Louradour
,
G. Bourg-Heckly
ENDOSCOPIC MICROSCOPY VI, 7893, 789309 (2011)
DOI: 10.1117/12.875440
ISSN: 0277-786X
Abstract
We are developing a non-linear fibered endomicroscope for imaging the extracellular matrix collagen and elastin fibrillar networks during bronchoscopy. As a proof of concept, laser pulses at the output of a standard 2 meter long single-mode fibre have been obtained with pulse duration of about 50 fs and pulse energy up to 50 uJ, using a specially designed grism line for the dispersion compensation. With these pulses, we performed a spectroscopic characterization of the non-linear endogenous signal, consisting of two-photon fluorescence and second harmonic generation, and originated from human pulmonary tissue of various thickness, both in forward and backward geometry of signal collection, with excitation at 830 nm.
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