Cortical Contractility Triggers a Stochastic Switch to Fast Amoeboid Cell Motility

V. Ruprecht , S. Wieser , A. Callan-Jones , M. Smutny , H. Morita , K. Sako , V. Barone , M. Ritsch-Marte , M. Sixt , R. Voituriez , C.P. Heisenberg

Bibtex , URL
CELL, 160, 4
Published 12 Feb. 2015
DOI: 10.1016/j.cell.2015.01.008
ISSN: 0092-8674


3D amoeboid cell migration is central to many developmental and disease-related processes such as cancer metastasis. Here, we identify a unique prototypic amoeboid cell migration mode in early zebrafish embryos, termed stable-bleb migration. Stable-bleb cells display an invariant polarized balloon-like shape with exceptional migration speed and persistence. Progenitor cells can be reversibly transformed into stable-bleb cells irrespective of their primary fate and motile characteristics by increasing myosin II activity through biochemical or mechanical stimuli. Using a combination of theory and experiments, we show that, in stable-bleb cells, cortical contractility fluctuations trigger a stochastic switch into amoeboid motility, and a positive feedback between cortical flows and gradients in contractility maintains stable-bleb cell polarization. We further show that rearward cortical flows drive stable-bleb cell migration in various adhesive and non-adhesive environments, unraveling a highly versatile amoeboid migration phenotype.

Cette publication est associée à :

Dynamique stochastique des systèmes réactifs et vivants