Working Together: Spatial Synchrony in the Force and Actin Dynamics of Podosome First Neighbors

A. Proag , A. Bouissou , T. Mangeat , R. Voituriez , P. Delobelle , C. Thibault , C. Vieu , I. Maridonneau-Parini , R. Poincloux

Bibtex , URL
ACS NANO, 9, 4
Published 01 Apr. 2015
DOI: 10.1021/nn506745r
ISSN: 1936-0851


Podosomes are mechanosensitive adhesion cell structures that are capable of applying protrusive forces onto the extracellular environment. We have recently developed a method dedicated to the evaluation of the nanoscale forces that podosomes generate to protrude into the extracellular matrix. It consists in measuring by atomic force microscopy (AFM) the nanometer deformations produced by macrophages on a compliant Formvar membrane and has been called protrusion force microscopy (PFM). Here we perform time-lapse PFM experiments and investigate spatial correlations of force dynamics between podosome pairs. We use an automated procedure based on finite element simulations that extends the analysis of PFM experimental data to take into account podosome architecture and organization. We show that protrusion force varies in a synchronous manner for podosome first neighbors, a result that correlates with phase synchrony of core F-actin temporal oscillations. This dynamic spatial coordination between podosomes suggests a short-range interaction that regulates their mechanical activity.

This publication is related to:

Stochastic dynamics of reactive and living systems