The brain is a complex structure comprising many different neuron populations making specific and long ranged polarized connections, or synapses.As such, neurons are ordered in exquisitely complex yet organized directional neuronal pathways. In an effort to optimize reconstruction of neuronal network, we have previously shown that fluidically isolated cell culture chambers separated by asymmetrical microstructures and micro-channels ("axon diode"), allow efficient unidirectional orientation of axons. Here, using these concept we reconstructs multi-components neuronal network in which specific neuronal subtypes are derived from rodent and human iPS cells. Using functional assays, optogenetic stimulation and calcium imaging, allowing to monitor the establishment of synaptic connection, glutamatergic transmission, and synchronous rhythmic activities, we characterized the development of the reconstructed neuronal networks and demonstrates their fully functionality. These Rodent and Human “brain on chip” plateforms are currently use to model progressive neurodegenerative syndroms and several application examples will be presented.