Innate control of actin nucleation determines two distinct migration behaviours in dendritic cells

P. Vargas , P. Maiuri , M. Bretou , P.J. Saez , P. Pierobon , M. Maurin , M. Chabaud , D. Lankar , D. Obino , E. Terriac , M. Raab , H.R. Thiam , T. Brocker , S.M. Kitchen-Goosen , A.S. Alberts , P. Sunareni , S. Xia , R. Li , R. Voituriez , M. Piel , A.M. Lennon-Dumenil

Bibtex , URL
Published 01 Jan. 2016
DOI: 10.1038/ncb3284
ISSN: 1465-7392


Dendritic cell (DC) migration in peripheral tissues serves two main functions: antigen sampling by immature DCs, and chemokine-guided migration towards lymphatic vessels (LVs) on maturation. These migratory events determine the efficiency of the adaptive immune response. Their regulation by the core cell locomotion machinery has not been determined. Here, we show that the migration of immature DCs depends on two main actin pools: a RhoA mDial-dependent actin pool located at their rear, which facilitates forward locomotion; and a Cdc42 Arp2/3-dependent actin pool present at their front, which limits migration but promotes antigen capture. Following TLR4 MyD88-induced maturation, Arp2/3-dependent actin enrichment at the cell front is markedly reduced. Consequently, mature DCs switch to a faster and more persistent mDial-dependent locomotion mode that facilitates chemotactic migration to LVs and lymph nodes. Thus, the differential use of actin-nucleating machineries optimizes the migration of immature and mature DCs according to their specific function.

Cette publication est associée à :

Dynamique stochastique des systèmes réactifs et vivants