Biomembrane plasticity and cellular function

The activity of the team was originally focused on the interaction of light with different classes of chromophores of biological and / or biomedical interest. Some of these chromophores exhibit a high triplet state quantum yields that enable reactive oxygen species (ROS) production. Such agents are defined as photosensitizer. We have already shown that it became possible to use these molecules to disturb the membrane models in a fine and controlled way. Our main goal today is to achieve greater levels of membrane complexity, to further explore the involved mechanisms, but also to strengthen their relevance to the biological point of view. Our aim is not only to change the composition of the model membranes on which we work, but mainly to better understand the relationship between membrane composition, its morphology and one specific biological activity. In this view, biomimetic membrane models such as proteo-liposome is used.


We are particularly interested in mitochondrial membranes. In eukaryote cells, mitochondria are key organelles for energy production and apoptosis that constantly fuse and divide. Two membranes constitute their envelope, with different levels of permeability. A hallmark of the inner mitochondrial membranes (IMM) is the presence of dynamic invaginations called “cristae”. These nanostructures (20-50 nm), rich in a specific mitochondrial phospholipid, the cardiolipin (CL), can change their shape and their density depending on energy demand. Their relative integrity is usually considered as a relevant indicator of the functional state of mitochondria, since cristae contain the OXPHOS complexes responsible for ATP production and they sequester Cytochrome C which release is involved in apoptotic signaling. Specific alterations of cristae morphology induce mitochondrion dysfunctions (in the Barth syndrome for example), and in many pathological situations, such alterations are also observed. Thus, our goal is to understand the role, the dynamic and the impact of cristae on mitochondrial function.  

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Using single-vesicle technologies to unravel the heterogeneity of extracellular vesicles - Nature Protocols
G. Bordanaba-Florit , F. Royo , S.G. Kruglik , J.M. Falcón-Pérez
  URL Full text PDF Bibtex doi:10.1038/s41596-021-00551-z


Lipid unsaturation properties govern the sensitivity of membranes to photo-induced oxidative stress - Biophysical Journal
A. Bour , S.G. Kruglik , M. Chabanon , P. Rangamani , N. Puff , S. Bonneau
  URL Full text PDF Bibtex doi:10.1016/j.bpj.2019.01.033
Raman tweezers microspectroscopy of circa 100 nm extracellular vesicles - Nanoscale
S.G. Kruglik , F. Royo , J.M. Guigner , L. Palomo , O. Seksek , P.Y. Turpin , I. Tatischeff , J.M. Falcon-Pérez
  URL Full text PDF Bibtex doi:10.1039/c8nr04677h


Assessment of the ability of poly(l-lysine)-poly(ethylene glycol) (PLL-PEG) hydrogels to support the growth of U87-MG and F98 glioma tumor cells - J. Appl. Polym. Sci.
E. Gontran , M. Juchaux , C. Deroulers , S. Kruglik , N. Huang , M. Badoual , O. Seksek
  URL Full text PDF Bibtex doi:10.1002/app.46287
Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part I. Theory - J. Phys. Chem. B
A. Hovan , S. Datta , S.G. Kruglik , D. Jancura , P. Miskovsky , G. Bano
  URL Full text PDF Bibtex doi:10.1021/acs.jpcb.8b00658


Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase - Proceedings of the National Academy of Sciences of the United States of America
V.G. Almendro-Vedia , P. Natale , M. Mell , S. Bonneau , F. Monroy , F. Joubert , I. López-Montero
  URL Full text PDF Bibtex doi:10.1073/pnas.1701207114
Structural changes and picosecond to second dynamics of cytochrome c in interaction with nitric oxide in ferrous and ferric redox states - Physical Chemistry Chemical Physics
S.G. Kruglik , B.K. Yoo , J.C. Lambry , J.L. Martin , M. Negrerie
  URL Full text PDF Bibtex doi:10.1039/c7cp02634j
Raman scattering-based multiconformational analysis for probing the structural differences between acetylcholine and acetylthiocholine. - Journal of Pharmaceutical and Biomedical Analysis
B. Hernández , P. Houzé , F. Pflüger , S.G. Kruglik , M. Ghomi
  URL Full text PDF Bibtex doi:10.1016/j.jpba.2017.01.049


From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide - Physical Chemistry Chemical Physics
B. Hernandez , E. Lopez-Tobar , S. Sanchez-Cortes , Y.M. Coic , B. Baron , A. Chenal , S.G. Kruglik , F. Pfluger , R. Cohen , M. Ghomi
  URL Full text PDF Bibtex doi:10.1039/c6cp04421b
Correlation between Mitochondrial Morphology and Functionality after Oxidative Stress - Biophys. J.
Z. Nadova , L. Lenkavska , A. Fragola , S. Bonneau , F. Sureau , P. Miskovsky
  URL Full text PDF Bibtex doi:
All characteristic Raman markers of tyrosine and tyrosinate originate from phenol ring fundamental vibrations - Journal of Raman Spectroscopy
B. Hernandez , Y.M. Coic , F. Pflueger , S.G. Kruglik , M. Ghomi
  URL Full text PDF Bibtex doi:10.1002/jrs.4776


Release kinetics of an amphiphilic photosensitizer by block-polymer nanoparticles - Int. J. Pharm.
R. Kerdous , F. Sureau , A. Bour , S. Bonneau
  URL Full text PDF Bibtex doi:10.1016/j.ijpharm.2015.09.032
Protonation-deprotonation and structural dynamics of antidiabetic drug metformin - Journal of Pharmaceutical and Biomedical Analysis
B. Hernandez , F. Pflueger , S.G. Kruglik , R. Cohen , M. Ghomi
  URL Full text PDF Bibtex doi:10.1016/j.jpba.2015.04.041
Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging - J. Fluoresc.
E. Kocisova , P. Praus , J. Bok , S. Bonneau , F. Sureau
  URL Full text PDF Bibtex doi:10.1007/s10895-015-1612-3
Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting - Nanomedicine: NBM
A.K.A. Silva , N. Luciani , F. Gazeau , K. Aubertin , S. Bonneau , C. Chauvierre , D. Letourneur , C. Wilhelm
  URL Full text PDF Bibtex doi:10.1016/j.nano.2014.11.009


Effect of PKC alpha expression on Bcl-2 phosphorylation and cell death by hypericin - Apoptosis
J. Joniova , M. Misuth, , F. Sureau , P. Miskovsky, , Z. Nadova,
  URL Full text PDF Bibtex doi:10.1007/s10495-014-1043-7
Low Concentration Structural Dynamics of Lanreotide and Somatostatin-14 - Biopolymers
B. Hernandez , Y.M. Coic , B. Baron , S.G. Kruglik , F. Pflueger , R. Cohen , C. Carelli , M. Ghomi
  URL Full text PDF Bibtex doi:10.1002/bip.22491
Disulfide linkage Raman markers: a reconsideration attempt - Journal of Raman Spectroscopy
B. Hernandez , F. Pflueger , E. Lopez-Tobar , S.G. Kruglik , J.V. Garcia-Ramos , S. Sanchez-Cortes , M. Ghomi
  URL Full text PDF Bibtex doi:10.1002/jrs.4521
Raman characterization of Avocado Sunblotch viroid and its response to external perturbations and self-cleavage - BMC Biophysics
G. Hui-Bon-Hoa , H. Kaddour , J. Vergne , S.G. Kruglik , M.C. Maurel
  URL Full text PDF Bibtex doi:10.1186/2046-1682-7-2


Impact of Photosensitizers Activation on Intracellular Trafficking and Viscosity - PLOS One
K. Aubertin , S. Bonneau , A.K.A. Silva , J.C. Bacri , F. Gallet , C. Wilhelm
  URL Full text PDF Bibtex doi:10.1371/journal.pone.0084850
Protonation-deprotonation of the glycine backbone as followed by Raman scattering and multiconformational analysis - Chemical Physics
B. Hernandez , F. Pflueger , S.G. Kruglik , M. Ghomi
  URL Full text PDF Bibtex doi:10.1016/j.chemphys.2013.08.008
Characteristic Raman lines of phenylalanine analyzed by a multiconformational approach - Journal of Raman Spectroscopy
B. Hernandez , F. Pflueger , S.G. Kruglik , M. Ghomi
  URL Full text PDF Bibtex doi:10.1002/jrs.4290
Magnetic and Photoresponsive Theranosomes: Translating Cell-Released Vesicles into Smart Nanovectors for Cancer Therapy - ACS Nano
A.K.A. Silva , J. Kolosnjaj-Tabi , S. Bonneau , I. Marangon , N. Boggetto , K. Aubertin , O. Clement , M.F. Bureau , N. Luciani , F. Gazeau , C. Wilhelm
  URL Full text PDF Bibtex doi:10.1021/nn400269x


Flavin Conjugates for Delivery of Peptide Nucleic Acids - ChemBioChem
F. Marlin, , P. Simon, , S. Bonneau , P. Alberti, , C. Cordier, , C. Boix, , L. Perrouault, , A. Fossey, , T. Saison-Behmoaras, , M. Fontecave, , C. Giovannangeli,
  URL Full text PDF Bibtex doi:10.1002/cbic.201200505
Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy - Journal of Extracellular Vesicles
I. Tatischeff , E. Larquet , J.M. Falcon-Pérez , P.Y. Turpin , S.G. Kruglik
  URL Full text PDF Bibtex doi:10.3402/jev.v1i0.19179
Octreotide Used for Probing the Type-II` beta-Turn CD and Raman Markers - Journal of Physical Chemistry B
B. Hernandez , Y.M. Coic , S.G. Kruglik , C. Carelli , R. Cohen , M. Ghomi
  URL Full text PDF Bibtex doi:10.1021/jp3036428
Absorption Band III Kinetics Probe the Picosecond Heme Iron Motion Triggered by Nitric Oxide Binding to Hemoglobin and Myoglobin - Journal of Physical Chemistry B
B.K. Yoo , S.G. Kruglik , I. Lamarre , J.L. Martin , M. Negrerie
  URL Full text PDF Bibtex doi:10.1021/jp300849y


Photo-dynamic induction of oxidative stress within cholesterol-containing membranes: Shape transitions and permeabilization - Biochem. Biophys. Acta - Biomembranes
R. Kerdous , J. Heuvingh , S. Bonneau
  URL Full text PDF Bibtex doi:10.1016/j.bbamem.2011.08.002


Interaction dynamics of hypericin with low-density lipoproteins and U87-MG cells - International Journal of Pharmaceutics
V. Huntosova , L. Alvarez , L. Bryndzova , Z. Nadova , D. Jancura , L. Buriankova , S. Bonneau , D. Brault , P. Miskovsky , F. Sureau
  URL Full text PDF Bibtex doi:10.1016/j.ijpharm.2010.01.010
Photosensitization of polymer vesicles: a multistep chemical process deciphered by micropipette manipulation - Soft Matter
E. Mabrouk , S. Bonneau , L. Jia , D. Cuvelier , M.H. Li , P. Nassoy
  URL Full text PDF Bibtex doi:10.1039/c002065f


Asymmetric Oxidation of Giant Vesicles Triggers Curvature-Associated Shape Transition and Permeabilization - Biophys. J.
J. Heuvingh , S. Bonneau
  URL Full text PDF Bibtex doi:10.1016/j.bpj.2009.08.056
Influence of surface energy distribution on neuritogenesis - Colloids Surf B Biointerfaces
G. Lamour , N. Journiac , S. Soues , S. Bonneau , P. Nassoy , A. Hamraoui
  URL Full text PDF Bibtex doi:10.1016/j.colsurfb.2009.04.006
Photosensitizing properties of chlorins in solution and in membrane-mimicking systems - Photochem Photobiol Sci.
H. Mojzisova , S. Bonneau , P. Maillard , K. Berg , D. Brault
  URL Full text PDF Bibtex doi:10.1039/b822269j


Membrane Deformation under Local pH Gradient: Mimicking Mitochondrial Cristae Dynamics - Biophys. J.
N. Khalifat , N. Puff , S. Bonneau , J.B. Fournier , M.I. Angelova
  URL Full text PDF Bibtex doi:10.1529/biophysj.108.136077


Cellular uptake and subcellular distribution of chlorin e6 as functions of pH and interactions with membranes and lipoproteins - Biochim Biophys Acta
H. Mojzisova , S. Bonneau , C. Vever-Bizet , D. Brault
  URL Full text PDF Bibtex doi:10.1016/j.bbamem.2007.07.002
Structural and physico-chemical determinants of the interactions of macrocyclic photosensitizers with cells - Eur Biophys J.
H. Mojzisova , S. Bonneau , D. Brault
  URL Full text PDF Bibtex doi:10.1007/s00249-007-0204-9
Tetrapyrrole-photosensitizers vectorization and plasma LDL: A physico-chemical approach - International Journal of Pharmaceutics
S. Bonneau , C. Vever-Bizet , H. Mojzisova , D. Brault
  URL Full text PDF Bibtex doi:10.1016/j.ijpharm.2007.06.019
The pH-dependent distribution of the photosensitizer chlorin e6 among plasma proteins and membranes: A physico-chemical approach - Biochim Biophys Acta
H. Mojzisova , S. Bonneau , C. Vever-Bizet , D. Brault
  URL Full text PDF Bibtex doi:10.1016/j.bbamem.2006.10.009