Biomembrane plasticity and cellular function
The activity of the team was originally focused on the interaction of light with different classes of chromophores of biological and / or biomedical interest. Some of these chromophores exhibit a high triplet state quantum yields that enable reactive oxygen species (ROS) production. Such agents are defined as photosensitizer. We have already shown that it became possible to use these molecules to disturb the membrane models in a fine and controlled way. Our main goal today is to achieve greater levels of membrane complexity, to further explore the involved mechanisms, but also to strengthen their relevance to the biological point of view. Our aim is not only to change the composition of the model membranes on which we work, but mainly to better understand the relationship between membrane composition, its morphology and one specific biological activity. In this view, biomimetic membrane models such as proteo-liposome is used.
We are particularly interested in mitochondrial membranes. In eukaryote cells, mitochondria are key organelles for energy production and apoptosis that constantly fuse and divide. Two membranes constitute their envelope, with different levels of permeability. A hallmark of the inner mitochondrial membranes (IMM) is the presence of dynamic invaginations called “cristae”. These nanostructures (20-50 nm), rich in a specific mitochondrial phospholipid, the cardiolipin (CL), can change their shape and their density depending on energy demand. Their relative integrity is usually considered as a relevant indicator of the functional state of mitochondria, since cristae contain the OXPHOS complexes responsible for ATP production and they sequester Cytochrome C which release is involved in apoptotic signaling. Specific alterations of cristae morphology induce mitochondrion dysfunctions (in the Barth syndrome for example), and in many pathological situations, such alterations are also observed. Thus, our goal is to understand the role, the dynamic and the impact of cristae on mitochondrial function.
Related pages
High-speed nanoscopy to decipher the real-time mitochondrial dynamics - Dyn@mitRelated job openings
2019
2018
Publications
2019
⊞ | Lipid unsaturation properties govern the sensitivity of membranes to photo-induced oxidative stress - Biophysical Journal |
⊞ | Raman tweezers microspectroscopy of circa 100 nm extracellular vesicles - Nanoscale |
URL | Full text PDF | Bibtex | doi:10.1039/c8nr04677h |
2018
⊞ | Assessment of the ability of poly(l-lysine)-poly(ethylene glycol) (PLL-PEG) hydrogels to support the growth of U87-MG and F98 glioma tumor cells - J. Appl. Polym. Sci. |
URL | Full text PDF | Bibtex | doi:10.1002/app.46287 |
⊞ | Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part I. Theory - J. Phys. Chem. B |
URL | Full text PDF | Bibtex | doi:10.1021/acs.jpcb.8b00658 |
⊞ | Phosphorescence Kinetics of Singlet Oxygen Produced by Photosensitization in Spherical Nanoparticles. Part II. The Case of Hypericin-Loaded Low-Density Lipoprotein Particles - J. Phys. Chem. B |
URL | Full text PDF | Bibtex | doi:10.1021/acs.jpcb.8b00659 |
2017
⊞ | Nonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthase - Proceedings of the National Academy of Sciences of the United States of America |
URL | Full text PDF | Bibtex | doi:10.1073/pnas.1701207114 |
⊞ | Structural changes and picosecond to second dynamics of cytochrome c in interaction with nitric oxide in ferrous and ferric redox states - Physical Chemistry Chemical Physics |
URL | Full text PDF | Bibtex | doi:10.1039/c7cp02634j |
⊞ | Photo-induced oxidation of bio-mimetic membranes : Giant pore openings and membrane defects - Eur. Biophys. J. Biophys. Lett. |
⊞ | Raman scattering-based multiconformational analysis for probing the structural differences between acetylcholine and acetylthiocholine. - Journal of Pharmaceutical and Biomedical Analysis |
2016
⊞ | From bulk to plasmonic nanoparticle surfaces: the behavior of two potent therapeutic peptides, octreotide and pasireotide - Physical Chemistry Chemical Physics |
⊞ | Berberine as a photosensitizing agent for antitumoral photodynamic therapy: Insights into its association to low density lipoproteins - Int. J. Pharm. |
⊞ | Correlation between Mitochondrial Morphology and Functionality after Oxidative Stress - Biophys. J. |
⊞ | All characteristic Raman markers of tyrosine and tyrosinate originate from phenol ring fundamental vibrations - Journal of Raman Spectroscopy |
2015
⊞ | Release kinetics of an amphiphilic photosensitizer by block-polymer nanoparticles - Int. J. Pharm. |
⊞ | Protonation-deprotonation and structural dynamics of antidiabetic drug metformin - Journal of Pharmaceutical and Biomedical Analysis |
⊞ | Intracellular Monitoring of AS1411 Aptamer by Time-Resolved Microspectrofluorimetry and Fluorescence Imaging - J. Fluoresc. |
⊞ | Combining magnetic nanoparticles with cell derived microvesicles for drug loading and targeting - Nanomedicine: NBM |
2014
⊞ | Disulfide linkage Raman markers: a reconsideration attempt - Journal of Raman Spectroscopy |
⊞ | Raman characterization of Avocado Sunblotch viroid and its response to external perturbations and self-cleavage - BMC Biophysics |
URL | Full text PDF | Bibtex | doi:10.1186/2046-1682-7-2 |
2013
⊞ | Protonation-deprotonation of the glycine backbone as followed by Raman scattering and multiconformational analysis - Chemical Physics |
⊞ | Characteristic Raman lines of phenylalanine analyzed by a multiconformational approach - Journal of Raman Spectroscopy |
2012
⊞ | Flavin Conjugates for Delivery of Peptide Nucleic Acids - ChemBioChem |
⊞ | Fast characterisation of cell-derived extracellular vesicles by nanoparticles tracking analysis, cryo-electron microscopy, and Raman tweezers microspectroscopy - Journal of Extracellular Vesicles |
URL | Full text PDF | Bibtex | doi:10.3402/jev.v1i0.19179 |
⊞ | Octreotide Used for Probing the Type-II` beta-Turn CD and Raman Markers - Journal of Physical Chemistry B |
⊞ | Absorption Band III Kinetics Probe the Picosecond Heme Iron Motion Triggered by Nitric Oxide Binding to Hemoglobin and Myoglobin - Journal of Physical Chemistry B |
URL | Full text PDF | Bibtex | doi:10.1021/jp300849y |
2011
⊞ | Photo-dynamic induction of oxidative stress within cholesterol-containing membranes: Shape transitions and permeabilization - Biochem. Biophys. Acta - Biomembranes |
⊞ | Hypericin incorporation and localization in fixed HeLa cells for various conditions of fixation and incubation - Photochem. Photobiol. Sciences |
2010
⊞ | Interaction dynamics of hypericin with low-density lipoproteins and U87-MG cells - International Journal of Pharmaceutics |
⊞ | Photosensitization of polymer vesicles: a multistep chemical process deciphered by micropipette manipulation - Soft Matter |
2009
⊞ | Asymmetric Oxidation of Giant Vesicles Triggers Curvature-Associated Shape Transition and Permeabilization - Biophys. J. |
⊞ | Influence of surface energy distribution on neuritogenesis - Colloids Surf B Biointerfaces |
⊞ | Photosensitizing properties of chlorins in solution and in membrane-mimicking systems - Photochem Photobiol Sci. |
2008
⊞ | Membrane Deformation under Local pH Gradient: Mimicking Mitochondrial Cristae Dynamics - Biophys. J. |
⊞ | Tetrapyrrole photosensitisers, determinants of subcellular localisation and mechanisms of photodynamic processes in therapeutic approaches - Expert Opinion on Therapeutic Patents |
2007
⊞ | Cellular uptake and subcellular distribution of chlorin e6 as functions of pH and interactions with membranes and lipoproteins - Biochim Biophys Acta |
⊞ | Structural and physico-chemical determinants of the interactions of macrocyclic photosensitizers with cells - Eur Biophys J. |
⊞ | Tetrapyrrole-photosensitizers vectorization and plasma LDL: A physico-chemical approach - International Journal of Pharmaceutics |