Cell division homeostasis is essential for long-term cell survival and for the ability of cells to adapt to fluctuating environments. Our work aims to understand how cells integrate internal and environmental cues to regulate proliferation, stress responses, and longevity.

To address these questions, we develop quantitative imaging approaches that allow us to track single yeast cell divisions over time in tightly controlled microenvironments. This experimental framework enables us to follow cell fate decisions across multiple generations.

Using yeast as a model system, I will first show how intrinsic division control mechanisms limit cellular lifespan and shape replicative aging. I will then describe how cells adapt their growth and division dynamics in response to oxidative stress, focusing on hydrogen peroxide as a model perturbation. Next, I will examine how nutrient availability modulates the balance between proliferation and survival under both basal and stress conditions.

Finally, I will present our most recent results and discuss ongoing and future directions aimed at understanding how these regulatory layers are integrated at the single-cell level.